Demethylcurcumin analogs, the highly potent compounds for antioxidation in G6PD normal and deficient subjects

Authors

  • Duangmanee Sanmun Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University
  • Chatchawan Changtam Division of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University
  • Sarawut Saichanma Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University
  • Sucha Chulsomlee Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University
  • Pornsuri Pongsuchart Division of Clinical Microscopy, Faculty of Medical Technology, Huachiew Chalermprakiet University
  • Apichart Suksamrarn Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University

Keywords:

antioxidant, curcuminoids, curcumin analog, red blood cell, G6PD deficiency

Abstract

Glucose-6-phosphate dehydrogenase (G6PD) deficiency can cause hemolytic anemia in response to oxidative stress. This study was undertaken to study the protective effect of natural curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) with curcuminoid analogs (di-O-demethylcurcumin and mono-O-demethylcurcumin) in healthy and G6PDdeficient red blood cells. After pretreatment blood with the test compounds, 0.75 mM sodium nitrite-oxidized hemolysate was determined for methemoglobin (metHb) formation. Concurrently, the released hemoglobin from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-challenged cells was measured for hemolysis. We found that most of the compounds reduced metHb formation and oxidative hemolysis in red cells. After pretreatment with curcuminoid analogs, the oxidized hemoglobins were lower than treated with natural compounds. Furthermore, this study showed that demethylcurcumin analogs certainly protect AAPH-induced red cell lysis. This study can be concluded that curcuminoid analogs with different phenol rings possibly have higher antioxidant activity in order to protect the oxidative stress condition, especially in G6PD deficiency.

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Published

2018-06-20

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Section

Research Articles