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This research investigated the efficacy of adjuvant (Montanide ISA 763 A VG) at concentrations of 0, 5, 10, 20 and 50% in formalin-killed Streptococcus agalactiae vaccine in Nile tilapia (Oreochromis niloticus Linn.). The results showed that adjuvant at 20 and 50% induced significantly higher protection (survival and RPS value) than other groups after three months of experimental trial (P < 0.05). However, only the antibody titer of 20% adjuvant was significantly higher than the control group (P < 0.05). The second experiment aimed to find the efficacy of a single vaccination with 10 and 20% adjuvant compared with non-adjuvant vaccine with a booster dose. The results showed that after the booster vaccination without adjuvant, the antibody titer was significantly higher than other treatments (P < 0.05). However, in terms of protection (survival and RPS), the vaccines with both 10 and 20% adjuvant showed similar efficacy to the booster vaccination until the third month of the trial (P > 0.05), and were significantly different from the vaccine without adjuvant (non-booster group) and from the control (non-vaccination) (P < 0.05). Thus, adjuvant vaccine was as effective as booster vaccination for the first three months of the four-month trial. Further study with the aim to extend protection with a single dose of adjuvant vaccine is being conducted.
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