Gene Silencing of Translationally Controlled Tumor Protein (TCTP)

Abstract

Background and Objective: The translationally controlled tumor protein (TCTP) overexpression was founded in many cancers indicating its role in tumorigenesis. We determined the role of TCTP on cell migration of human cholangiocarcinoma (CCA) cell line by RNA interference approach.

Method: The M139 human CCA cell line with high-endogenous TCTP expression was used in this experiment. The effect of small interference RNA (siRNA) based knocking down of TCTP gene on cell migration was evaluated.

Results: The result showed M139 cells transfection with si_TCTP reduced the expression of TCTP protein. The decreased TCTP protein level was associated with decreased cell migration of M139 cells.

Conclusion: This study is the first observation for elucidating the role of TCTP in CCA metastasis. Gene silencing of TCTP by siRNA inhibits cell migration of M139 CCA cell line suggesting that TCTP might be a new targeting molecule in CCA progression.