Genetic Relationship Assessment and Identification of Strap-Leaf Paphiopedilum Using HAT-RAPD Markers

Authors

  • Pornprapa Siritheptawee Department of Biotechnology, Faculty of Science and Technology Thammasat University, Pathum Thani 12120, Thailand
  • Somchit Damrianant Department of Biotechnology, Faculty of Science and Technology Thammasat University, Pathum Thani 12120, Thailand
  • Theerachai Thanananta Department of Biotechnology, Faculty of Science and Technology Thammasat University, Pathum Thani 12120, Thailand
  • Narumol Thanananta Faculty of Science and Technology, Valaya Alongkorn Rajabhat University under Royal Patronage, Pathum Thani 13180, Thailand

Abstract

 High annealing temperature - random amplified polymorphic DNA (HAT-RAPD) marker was used to identify and investigate the genetic relationship among 15 Paphiopedilum species of Venus slipper. The total of 72 primers was screened and 16 primers could be used for DNA amplification with clear amplified products to construct DNA fingerprints. The total of 248 polymorphic bands was found. A dendrogram, which constructed from the polymorphic bands using UPGMA by the NTSYS program, showed genetic similarities among 15 Paphiopedilum species with similarity coefficients ranging from 0.252 to 0.624. The orchids were classified into 3 clusters. These results indicated that the HAT-RAPD markers are capable to specify Paphiopedilum, and can be used in the breeding program and for genetic resource conservation in the future. 

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Published

2018-05-21

How to Cite

Siritheptawee, P., Damrianant, S., Thanananta, T., & Thanananta, N. (2018). Genetic Relationship Assessment and Identification of Strap-Leaf Paphiopedilum Using HAT-RAPD Markers. Science & Technology Asia, 23(1), 17–22. Retrieved from https://ph02.tci-thaijo.org/index.php/SciTechAsia/article/view/124816

Issue

Vol.23 No.1 (January-March 2018)

Section

Biological sciences