Pollen viability and seed set of interspecific hybrids between J. curcas × J. integerrima

Authors

  • Kitiya - Amkul Plant Breeding Program, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom
  • Malee - Panngam Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom
  • Patcharin - Tanya Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok.
  • Peerasak - Srinives Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom. Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok.
  • Kularb - Laosatit Postdoctoral Fellow, the project on Breeding to Accelerate Domestication of Novel Jatropha for Fuel and Feeds, Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom

Keywords:

Interspecific hybrids, acetocarmine, staining method, viability test

Abstract

Interspecific hybrids between Jatropha curcas × J. integerrima are potential genetic resources for jatropha improvement. However, their pollen is normally partially sterile. This study was conducted to determine pollen viability status and seed set of the hybrids. Pollen viability was estimated by staining with 1% acetocarmine. The results showed that all interspecific hybrids had pollen viability falling between their parents. The pollen viability of interspecific hybrid derived from a cross between J. curcas (high phorbol esters accession) × J. integerrima ranged from 47 % to 89 % with an average of 72 %. Similarly the viability of the hybrid J. curcas (low phorbol esters accession) × J. integerrima ranged from 44 % to 90 % with an average of 73 %. The hybrid with low pollen viability also set less seeds. This information is useful for plant breeders to select the pollen donor in interspecific breeding programs. 

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Published

2016-06-27

Issue

Section

Short Communications