Free radical Scavenging and Anti-mutagenic activities of constituents from ArdisiaellipticaThunb. ripe fruits

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Methin Phadungkit

Abstract

ArdisiaellipticaThunb.is a Thai medicinal plant in the Myrsinaceae family. The fruits of this plant are traditionallyused as anti-diarrheal agent. The current study aimed to test free radical scavenging and antimutagenic activities of the A. elliptica ripe fruit extracts. The dry fruits of this plant were extracted by the maceration method using hexane, dichloromethane and methanol as solvents. The free radical scavenging activity was performed using the DPPH (2,2-diphenyl- 1-picrylhydrazyl) assay. The antimutagenic actions were tested using the Ames test in Salmonella typhimurium TA98 and TA100 strains. The bioactive compound from the active extract was investigated using the chromatographic and the spectroscopic techniques. The results showed that the methanol extract possessed the highest free radical scavenging activitywith IC50 value of 8.87±0.24 μg/mL and the dichloromethane extract showed the highest antimutagenic capacity both in TA98 and TA100 strains. The bioactive compound isolated from the dichloromethane extract was proposed as syringic acid. The isolated compound exhibited the strongest free radical scavenging and antimutagenic activities when compared withthe herbal extracts. From this in vitro study, it can be concluded that the A. ellipticaextractsand its chemical component have excellent potential for further study as health products such as dietary supplements.

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บทความวิจัย