Chemical Profiling of an Antipyretic Drug, Thai Herbal Harak Formula, by Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

Authors

  • Patcharamon Seubnooch Department of Pharmacology
  • Jantanee Wattanarangsan Department of Pharmacology
  • Onusa Thamsermsang enter of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Suksalin Booranasubkajorn Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Tawee Laohapand Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
  • Pravit Akarasereenont Department of Pharmacology

Abstract

Objective: To isolate and identify chemical compounds in Harak, a Thai herbal formula widely used as an antipyretic drug in Thailand.
Methods: Methanol extraction of the Harak formula and its five herbal components were separated by Ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. This method has
been developed for “untargeted” profiling of this formula and its components. An online database was used to identify chemical compounds by comparing its empirical molecular formula, isotope pattern, and fragmentation pattern.
Results: Nineteen chemical constituents were found from positive and negative electrospray ionization (ESI) modes. These compounds included flavonoids, hesperitine, and iso-corydine, which are known to possess antioxidant and
anticancer activity. Moreover, data from the principle component analysis (PCA) score plot of positive and negative ESI modes showed that the chemical constituents of Thai herbal Harak formula were similar to those found in Ficus
racemosa Linn. and Capparis micracantha DC.
Conclusion: Under this optimization method, nineteen chemical constituents including phenolic and flavonoids were characterized in both positive and negative ESI mode.

Downloads

Published

07-06-2018

How to Cite

Seubnooch, P., Wattanarangsan, J., Thamsermsang, O., Booranasubkajorn, S., Laohapand, T., & Akarasereenont, P. (2018). Chemical Profiling of an Antipyretic Drug, Thai Herbal Harak Formula, by Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry. Siriraj Medical Journal, 70(2), 159–168. Retrieved from https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127514

Issue

Vol. 70 No. 2 (2018): March-April

Section

Original Article

License

Users are free to share, copy, and redistribute all articles published in the Siriraj Medical Journal (SMJ) in any medium or format as long as you follow the following terms:

  • Attribution — You must give appropriate credit, provide a link to the material, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the publisher endorses you or your use.
  • NonCommercial — You may not use the material for commercial purposes.
  • NoDerivatives — If you remix, transform, or build upon the material, you may not distribute the modified material.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.